Defending champion and world number one Serena Williams was knocked out of the French Open by Spain’s Garbine Muguruza as the American star stumbled to her second worst Grand Slam defeat.
The top seed lost 6-2 6-2 in the second round to the world number 35 with her defeat coming just an hour after sister Venus also went out, 2-6 6-3 6-4 to Slovak teenager Anna Schmiedlova.
It was the earliest exit for a defending champion at Roland Garros since 2005 when Anastasia Myskina of Russia was eliminated in the first round.
With Li Na losing in the first round on Tuesday, Serena’s defeat marks the first time in the Open Era that the top two seeds had failed to get to the third round at a major.
Wednesday’s defeat was Serena’s worst at a Grand Slam in terms of games won and was only the second time she had been beaten at a major in the round of 64.
The last time that happened was on her debut at the 1998 Australian Open.
“Nothing worked today,” said Serena, who suffered her only Grand Slam first-round loss in Paris in 2012.
“I just couldn’t serve. But you can’t be on form every day. I hate to be off at a Grand Slam but it happens, it’s not the end of the world.”
Serena’s defeat was good news for Maria Sharapova who is now the overwhelming title favourite.
The Russian seventh seed, the champion in 2012, responded to the challenge by beating Bulgaria’s Tsvetana Pironkova 7-5 6-2.
As the American sisters exited, compatriot 18-year-old Taylor Townsend gave her country a glimpse of a bright future when the world number 205, making her Grand Slam debut, knocked out French 20th seed Alize Cornet, 6-4 4-6 6-4.
Third seed Agnieszka Radwanska of Poland, the highest-ranked woman left in the draw, reached the third round with a 6-3 6-4 win over Karolina Pliskova of the Czech Republic and next faces Croatia’s Ajla Tomljanovic.
The men’s tournament was sedate by comparison.
Second seed Novak Djokovic, the 2012 runner-up who needs a French Open title to complete a career Grand Slam, enjoyed a 6-1 6-4 6-2 win over Jeremy Chardy of France to reach the last 32.
Djokovic took his record over the world number 42 to 9-0.
Roger Federer, the 2009 champion and fourth seed, also reached the third round with a 6-3 6-4 6-4 win over Argentine qualifier Diego Sebastian Schwartzman.
It was Federer’s 60th win at Roland Garros as he goes on to face Russian 31st seed Dmitry Tursunov for a place in the last 16.
Also progressing were Czech sixth seed Tomas Berdych who beat Alexsandr Nedovyesov of Kazakhstan 6-7 (4/7) 6-4 7-5, 6-3 and Milos Raonic, the eighth-seeded Canadian who defeated Jiri Vesely of the Czech Republic, 7-6 (7/4) 6-4 6-1.